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KMID : 1134820170460010026
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 1 p.26 ~ p.33
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Immune-Enhancing Effects of Polysaccharides Isolated from Phellinus linteus Mycelium on Mori ramul
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Park Hye-Mi
Hong Joo-Heon
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Abstract
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The objective of this study was to examine the immune-enhancing effects of polysaccharides isolated from Phellinus linteus mycelium on Mori ramulus. Crude polysaccharides were isolated by pressurized extraction (121°C, 1.2 kgf/cm2, 3 h), ethanol precipitation, and lyophilization. In addition, crude polysaccharides were further fractionated into unabsorbed fractions (PF-1, fraction No. 3∼15) and absorbed fractions (PF-2, fraction No. 24∼33) by DEAE-sepharose CL-6B column chromatography in order to isolate immune-regulating polysaccharides. The major constituents in PF-1 and PF-2 were total sugar (75.51% and 52.38%), total protein (1.63% and 8.41%), uronic acid (17.53% and 15.04%), and β-glucan (28.33% and 25.04%), respectively. PF-1 increased production of nitric oxide (NO) and cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner. The mRNA expression levels of inducible NO synthetase, cyclooxygenase-2, TNF-α, and IL-6 markedly increased as determined by polymerase chain reaction analysis. The above data led us to conclude that macrophage activation of purified polysaccharides was higher than that of crude polysaccharides. The polysaccharides isolated from P. linteus mycelium on M. ramulus investigated herein are useful as natural immune-enhancing agents.
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KEYWORD
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Phellinus linteus mycelium, polysaccharide, immune enhancing, nitric oxide, cytokine production
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